Mouse Leukotriene A4 Hydrolase ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Leukotriene A4 Hydrolase in biofluid samples, such as serum, plasma, tissue homogenates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . LTA4H present in samples reacts with an anti-Mouse LTA4H capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-LTA4H detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active LTA4H in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse LTA4H. Detection Target: Mouse Leukotriene A4 Hydrolase Reactivity: Mouse Range: 0.156-10ng/mL Sensitivity: 0.051ng/mL Storage Conditions: Store all components at 4┬░C This Mouse LTA4H activity assay is for the quantitative determination of Leukotriene A4 Hydrolase in samples including serum, plasma, tissue homogenates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.